| Number | Gene Name | Protein Name | Primary Tissue | Median Essentiality Score | Number of Cell Lines |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Skin | -3.732 | 4 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Head and Neck | -2.794 | 34 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Esophagus | -2.733 | 26 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Kidney | -2.644 | 3 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Bone | -2.57 | 12 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Central Nervous System | -2.397 | 33 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Haematopoietic and Lymphoid | -2.396 | 14 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Large Intestine | -2.313 | 30 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Peripheral Nervous System | -2.263 | 17 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Ovary | -2.107 | 31 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Endometrium | -2.087 | 8 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Lung | -2.015 | 42 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Stomach | -1.835 | 14 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Pancreas | -1.729 | 23 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Prostate | -1.438 | 3 |
| 1 | MARS | Methionine--tRNA ligase, cytoplasmic | Breast | -1.241 | 25 |
| 2 | VARS | Valine--tRNA ligase | Skin | -3.61 | 4 |
| 2 | VARS | Valine--tRNA ligase | Esophagus | -3.227 | 26 |
| 2 | VARS | Valine--tRNA ligase | Head and Neck | -2.997 | 34 |
| 2 | VARS | Valine--tRNA ligase | Large Intestine | -2.764 | 30 |
| 2 | VARS | Valine--tRNA ligase | Bone | -2.432 | 12 |
| 2 | VARS | Valine--tRNA ligase | Central Nervous System | -2.4 | 33 |
| 2 | VARS | Valine--tRNA ligase | Kidney | -2.307 | 3 |
| 2 | VARS | Valine--tRNA ligase | Lung | -2.285 | 42 |
| 2 | VARS | Valine--tRNA ligase | Stomach | -2.28 | 14 |
| 2 | VARS | Valine--tRNA ligase | Haematopoietic and Lymphoid | -2.277 | 14 |
| 2 | VARS | Valine--tRNA ligase | Endometrium | -2.167 | 8 |
| 2 | VARS | Valine--tRNA ligase | Ovary | -2.149 | 31 |
| 2 | VARS | Valine--tRNA ligase | Pancreas | -2.076 | 23 |
| 2 | VARS | Valine--tRNA ligase | Peripheral Nervous System | -2.034 | 17 |
| 2 | VARS | Valine--tRNA ligase | Prostate | -1.944 | 3 |
| 2 | VARS | Valine--tRNA ligase | Breast | -1.344 | 25 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Bone | -2.958 | 12 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Skin | -2.849 | 4 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Head and Neck | -2.64 | 34 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Esophagus | -2.451 | 26 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Central Nervous System | -2.445 | 33 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Large Intestine | -2.323 | 30 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Ovary | -2.192 | 31 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Haematopoietic and Lymphoid | -2.093 | 14 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Peripheral Nervous System | -2.072 | 17 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Endometrium | -1.981 | 8 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Lung | -1.889 | 42 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Stomach | -1.779 | 14 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Prostate | -1.764 | 3 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Pancreas | -1.762 | 23 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Kidney | -1.625 | 3 |
| 3 | EPRS | Bifunctional glutamate/proline--tRNA ligase | Breast | -1.44 | 25 |